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cd56 myoblasts  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd56 myoblasts
    Cd56 Myoblasts, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd56+myoblasts/PSA-NCAM+Antibody%2C+anti-human%2Fmouse%2Frat/pm40846977-82-77-88
    Average 96 stars, based on 42 article reviews
    cd56 myoblasts - by Bioz Stars, 2026-09
    96/100 stars

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    Incubation:

    Article Title: NAD + dyshomeostasis in RYR1-related myopathies.
    Article Snippet: .. The tissue fragment solution was transferred to a T-25 tissue culture flask (ThermoFisher #169,900) and left to adhere at room temperature for 20 min. Once adhered, 5 mL growth medium was added to the flask and the tissue was incubated for 7 days at 37 ̊C and 5% CO2 with a 50% media exchange every 48 h. Once at 90% confluency, primary cultures comprising myoblasts and fibroblasts were split using 5 mL 0.04% trypsin and enriched for CD56+ myoblasts by magnetic bead cell sorting per the manufacturer’s instructions (Miltenyi Biotec #130–097-042). ..

    Article Title: NAD + dyshomeostasis in RYR1 -related myopathies
    Article Snippet: .. The tissue fragment solution was transferred to a T-25 tissue culture flask (ThermoFisher #169,900) and left to adhere at room temperature for 20 min. Once adhered, 5 mL growth medium was added to the flask and the tissue was incubated for 7 days at 37 ̊C and 5% CO 2 with a 50% media exchange every 48 h. Once at 90% confluency, primary cultures comprising myoblasts and fibroblasts were split using 5 mL 0.04% trypsin and enriched for CD56 + myoblasts by magnetic bead cell sorting per the manufacturer’s instructions (Miltenyi Biotec #130–097-042). ..

    Article Title: Single Cell Analysis Identifies the miRNA Expression Profile of a Subpopulation of Muscle Precursor Cells Unique to Humans With Type 2 Diabetes
    Article Snippet: .. Cells grown to ∼50% confluency in a 10 cm culture dish were incubated with Human CD56 primary antibody conjugated magnetic microbeads (Miltenyi Biotec) at 4°C for 30 min. CD56+ myoblasts were filtered from the bulk population using a magnetic cell separator (Miltenyi Biotec) according to the manufacturer’s instructions (Miltenyi Biotec). .. Single cell capture, specific reverse transcription of miRNAs, and cDNA pre-amplification were performed using the Fluidigm ® C1 TM System.

    FACS:

    Article Title: NAD + dyshomeostasis in RYR1-related myopathies.
    Article Snippet: .. The tissue fragment solution was transferred to a T-25 tissue culture flask (ThermoFisher #169,900) and left to adhere at room temperature for 20 min. Once adhered, 5 mL growth medium was added to the flask and the tissue was incubated for 7 days at 37 ̊C and 5% CO2 with a 50% media exchange every 48 h. Once at 90% confluency, primary cultures comprising myoblasts and fibroblasts were split using 5 mL 0.04% trypsin and enriched for CD56+ myoblasts by magnetic bead cell sorting per the manufacturer’s instructions (Miltenyi Biotec #130–097-042). ..

    Article Title: Compromised mitochondrial quality control triggers lipin1-related rhabdomyolysis
    Article Snippet: .. CD56 + myoblasts were isolated by magnetic cell sorting using CD56 MicroBeads (Miltenyi Biotec) and routinely maintained in F10 medium supplemented with 20% Fetal Bovine Serum (FBS) both from Thermo Fisher Scientific on gelatin (Sigma Aldrich) coated tissue culture flasks. .. After magnetic sorting, purity of cells was assessed by flow cytometry using anti-CD56 Allophycocyanin conjugated antibody (BD Biosciences).

    Article Title: NAD + dyshomeostasis in RYR1 -related myopathies
    Article Snippet: .. The tissue fragment solution was transferred to a T-25 tissue culture flask (ThermoFisher #169,900) and left to adhere at room temperature for 20 min. Once adhered, 5 mL growth medium was added to the flask and the tissue was incubated for 7 days at 37 ̊C and 5% CO 2 with a 50% media exchange every 48 h. Once at 90% confluency, primary cultures comprising myoblasts and fibroblasts were split using 5 mL 0.04% trypsin and enriched for CD56 + myoblasts by magnetic bead cell sorting per the manufacturer’s instructions (Miltenyi Biotec #130–097-042). ..

    Isolation:

    Article Title: Compromised mitochondrial quality control triggers lipin1-related rhabdomyolysis
    Article Snippet: .. CD56 + myoblasts were isolated by magnetic cell sorting using CD56 MicroBeads (Miltenyi Biotec) and routinely maintained in F10 medium supplemented with 20% Fetal Bovine Serum (FBS) both from Thermo Fisher Scientific on gelatin (Sigma Aldrich) coated tissue culture flasks. .. After magnetic sorting, purity of cells was assessed by flow cytometry using anti-CD56 Allophycocyanin conjugated antibody (BD Biosciences).

    Magnetic Cell Separation:

    Article Title: Microphysiological system for studying contractile differences in young, active, and old, sedentary adult derived skeletal muscle cells
    Article Snippet: .. Mononuclear myoblasts were immunopurified using the mouse monoclonal 5.1H11 anti‐CD56 antibody (5.1H11 was deposited to the DSHB by Blau, H.M./Walsh, F.S. (DSHB Hybridoma Bank, Iowa City, IA) and CD56 + myoblasts were enriched using MACS magnetic bead technology (Miltenyi Biotec, Auburn, CA), according to the manufacturer's protocol. ..



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    Analysis of <t>CD56-</t> cells treated with ( A ) LiCl (controls, n = 2 and LGMDR1 patients, n = 3) and ( B ) Tideglusib and VP0.7 (controls, n = 3 and LGMDR1 patients, n = 3).
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    Image Search Results


    Analysis of CD56- cells treated with ( A ) LiCl (controls, n = 2 and LGMDR1 patients, n = 3) and ( B ) Tideglusib and VP0.7 (controls, n = 3 and LGMDR1 patients, n = 3).

    Journal: International Journal of Molecular Sciences

    Article Title: Allosteric Modulation of GSK-3β as a New Therapeutic Approach in Limb Girdle Muscular Dystrophy R1 Calpain 3-Related

    doi: 10.3390/ijms22147367

    Figure Lengend Snippet: Analysis of CD56- cells treated with ( A ) LiCl (controls, n = 2 and LGMDR1 patients, n = 3) and ( B ) Tideglusib and VP0.7 (controls, n = 3 and LGMDR1 patients, n = 3).

    Article Snippet: To obtain highly purified myoblasts (CD56+) and CD56- cells, primary cultures were sorted by immunomagnetic selection based on the presence of the early cell surface marker CD56 (separator and reagents from Miltenyi Biotec, Bergisch Gladbach, Germany).

    Techniques:

    Muscle and myoblast samples from healthy controls and LGMDR1 patients.

    Journal: International Journal of Molecular Sciences

    Article Title: Allosteric Modulation of GSK-3β as a New Therapeutic Approach in Limb Girdle Muscular Dystrophy R1 Calpain 3-Related

    doi: 10.3390/ijms22147367

    Figure Lengend Snippet: Muscle and myoblast samples from healthy controls and LGMDR1 patients.

    Article Snippet: To obtain highly purified myoblasts (CD56+) and CD56- cells, primary cultures were sorted by immunomagnetic selection based on the presence of the early cell surface marker CD56 (separator and reagents from Miltenyi Biotec, Bergisch Gladbach, Germany).

    Techniques: Functional Assay, Mutagenesis, Control