cd56 myoblasts (Miltenyi Biotec)
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Cd56 Myoblasts, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd56+myoblasts/PSA-NCAM+Antibody%2C+anti-human%2Fmouse%2Frat/pm40846977-82-77-88
Average 96 stars, based on 42 article reviews
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Incubation:Article Title: NAD + dyshomeostasis in RYR1-related myopathies. Article Snippet: .. The tissue fragment solution was transferred to a T-25 tissue culture flask (ThermoFisher #169,900) and left to adhere at room temperature for 20 min. Once adhered, 5 mL growth medium was added to the flask and the tissue was incubated for 7 days at 37 ̊C and 5% CO2 with a 50% media exchange every 48 h. Once at 90% confluency, primary cultures comprising myoblasts and fibroblasts were split using 5 mL 0.04% trypsin and enriched for Article Title: NAD + dyshomeostasis in RYR1 -related myopathies Article Snippet: .. The tissue fragment solution was transferred to a T-25 tissue culture flask (ThermoFisher #169,900) and left to adhere at room temperature for 20 min. Once adhered, 5 mL growth medium was added to the flask and the tissue was incubated for 7 days at 37 ̊C and 5% CO 2 with a 50% media exchange every 48 h. Once at 90% confluency, primary cultures comprising myoblasts and fibroblasts were split using 5 mL 0.04% trypsin and enriched for Article Title: Single Cell Analysis Identifies the miRNA Expression Profile of a Subpopulation of Muscle Precursor Cells Unique to Humans With Type 2 Diabetes Article Snippet: .. Cells grown to ∼50% confluency in a 10 cm culture dish were incubated with Human CD56 primary antibody conjugated magnetic microbeads (Miltenyi Biotec) at 4°C for 30 min. FACS:Article Title: NAD + dyshomeostasis in RYR1-related myopathies. Article Snippet: .. The tissue fragment solution was transferred to a T-25 tissue culture flask (ThermoFisher #169,900) and left to adhere at room temperature for 20 min. Once adhered, 5 mL growth medium was added to the flask and the tissue was incubated for 7 days at 37 ̊C and 5% CO2 with a 50% media exchange every 48 h. Once at 90% confluency, primary cultures comprising myoblasts and fibroblasts were split using 5 mL 0.04% trypsin and enriched for Article Title: Compromised mitochondrial quality control triggers lipin1-related rhabdomyolysis Article Snippet: .. Article Title: NAD + dyshomeostasis in RYR1 -related myopathies Article Snippet: .. The tissue fragment solution was transferred to a T-25 tissue culture flask (ThermoFisher #169,900) and left to adhere at room temperature for 20 min. Once adhered, 5 mL growth medium was added to the flask and the tissue was incubated for 7 days at 37 ̊C and 5% CO 2 with a 50% media exchange every 48 h. Once at 90% confluency, primary cultures comprising myoblasts and fibroblasts were split using 5 mL 0.04% trypsin and enriched for Isolation:Article Title: Compromised mitochondrial quality control triggers lipin1-related rhabdomyolysis Article Snippet: .. Magnetic Cell Separation:Article Title: Microphysiological system for studying contractile differences in young, active, and old, sedentary adult derived skeletal muscle cells Article Snippet: .. Mononuclear myoblasts were immunopurified using the mouse monoclonal 5.1H11 anti‐CD56 antibody (5.1H11 was deposited to the DSHB by Blau, H.M./Walsh, F.S. (DSHB Hybridoma Bank, Iowa City, IA) and |
